A Novel L-ascorbate Peroxidase 6 Gene, , Plays an Important Role in the Regulation of Response to Biotic and Abiotic Stresses in Sugarcane.

Clicks: 459
ID: 68102
2017
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #12 of 187 articles by views in Frontiers in plant science

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 187 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The L-ascorbate peroxidase 6 gene () is one of the most important genes for scavenging HO and plays a vital role in plant resistance to environmental stresses. In this study, a novel gene (GenBank Accession No. KT907352) was obtained from a sugarcane variety (ROC22). Bioinformatics analysis showed that ScAPX6 has a cDNA length of 1,086 bp and encoded 333 amino acid residues. Subcellular localization confirmed that ScAPX6 was located in the chloroplast. Enhanced growth of BL21 cells that expressed ScAPX6 showed high tolerance under copper (Cu) stress. Real-time quantitative PCR analysis revealed that was constitutively expressed wherein with the highest expression levels in sugarcane pith and leaf and the lowest in the root. was down-regulated by salicylic acid (SA), hydrogen peroxide (HO), polyethylene glycol (PEG) and sodium chloride (NaCl) stimuli. Interestingly, it was significantly up-regulated under the stresses of abscisic acid (ABA) and methyl jasmonate (MeJA) wherein with the highest inducible expression levels at 6 h at 6.0- and 70.0-times higher, respectively than that of control. Overexpression of in leaves enhanced the resistance to the infection of tobacco pathogens and var. . These results implied that might positively respond to ABA, MeJA, and Cu, but might negatively respond to the stresses of SA, HO, PEG, and NaCl. Keeping in view the current investigation, could be associated with the hypersensitive response (HR) or immunity of sugarcane, which will provide a baseline for the function identification of sugarcane .
Reference Key
liu2017afrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liu, Feng;Huang, Ning;Wang, Ling;Ling, Hui;Sun, Tingting;Ahmad, Waqar;Muhammad, Khushi;Guo, Jinxin;Xu, Liping;Gao, Shiwu;Que, Youxiong;Su, Yachun;
Journal Frontiers in plant science
Year 2017
DOI
10.3389/fpls.2017.02262
URL
Keywords
subcellular localization sugarcane l-ascorbate peroxidase 6 gene biotic and abiotic stresses transient overexpression

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.