CaWRKY22 Acts as a Positive Regulator in Pepper Response to by Constituting Networks with CaWRKY6, CaWRKY27, CaWRKY40, and CaWRKY58.

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ID: 68100
2018
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Abstract
The WRKY web, which is comprised of a subset of WRKY transcription factors (TFs), plays a crucial role in the regulation of plant immunity, however, the mode of organization and operation of this network remains obscure, especially in non-model plants such as pepper (). Herein, , a member of a subgroup of IIe WRKY proteins from pepper, was functionally characterized in pepper immunity against . CaWRKY22 was found to target the nuclei, and its transcript level was significantly upregulated by inoculation (RSI) and exogenously applied salicylic acid (SA), Methyl jasmonate (MeJA), or ethephon (ETH). Loss-of-function , caused by virus-induced gene silencing (VIGS), enhanced pepper’s susceptibility to RSI. In addition, the silencing of perturbed the hypersensitive response (HR)-like cell death elicited by RSI and downregulated defense-related genes including , , , , , , and . CaWRKY22 was found to directly bind to the promoters of , , and by chromatin immuno-precipitation (ChIP) analysis. Contrastingly, transient overexpression of in pepper leaves triggered significant HR-like cell death and upregulated the tested immunity associated maker genes. Moreover, the transient overexpression of upregulated the expression of and while it downregulated of the expression of . Conversely, the transient overexpression of , , and upregulated the expression of , while transient overexpression of downregulated the transcript levels of . These data collectively recommend the role of CaWRKY22 as a positive regulator of pepper immunity against , which is regulated by signaling synergistically mediated by SA, jasmonic acid (JA), and ethylene (ET), integrating into WRKY networks with WRKY TFs including CaWRKY6, CaWRKY27, CaWRKY40, and CaWRKY58.
Reference Key
hussain2018cawrky22international Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hussain, Ansar;Li, Xia;Weng, Yahong;Liu, Zhiqin;Ashraf, Muhammad Furqan;Noman, Ali;Yang, Sheng;Ifnan, Muhammad;Qiu, Shanshan;Yang, Yingjie;Guan, Deyi;He, Shuilin;
Journal International journal of molecular sciences
Year 2018
DOI
E1426
URL
Keywords
immunity capsicum annuum cawrky22 ralstonia solanacearum wrky networks

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