Provitamin A Biofortification of Durum Wheat through a TILLING Approach.

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ID: 67527
2019
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Abstract
Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a "block strategy" perspective. The null mutant genotype showed a strong reduction in the expression of the gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat.
Reference Key
sestili2019provitamininternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Sestili, Francesco;Garcia-Molina, Maria Dolores;Gambacorta, Gianluca;Beleggia, Romina;Botticella, Ermelinda;De Vita, Pasquale;Savatin, Daniel Valentin;Masci, Stefania;Lafiandra, Domenico;
Journal International journal of molecular sciences
Year 2019
DOI
E5703
URL
Keywords
vitamin a deficiency biofortification β-carotene durum wheat tilling

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