Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.

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ID: 66754
2018
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Abstract
Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells.Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate.Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine.
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Authors Hua, Tao;Zhang, Xuehua;Tang, Bo;Chang, Chen;Liu, Guoyang;Feng, Lei;Yu, Yang;Zhang, Daohua;Hou, Jibo;
Journal BMC veterinary research
Year 2018
DOI
10.1186/s12917-018-1457-5
URL
http://link.springer.com/article/10.1186/s12917-018-1457-5
Keywords
viral infection pcv2 cellular morphologic change nonionic surfactant tween-20

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