Uptake and metabolism of β-apo-8'-carotenal, β-apo-10'-carotenal, and β-apo-13-carotenone in Caco-2 cells.

Clicks: 315
ID: 66746
2019
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Abstract
β-Apocarotenoids are eccentric cleavage products of carotenoids formed by chemical and enzymatic oxidations. They occur in foods containing carotenoids and thus might be directly absorbed from the diet. However, there is limited information about their intestinal absorption. The present research examined the kinetics of uptake and metabolism of β-apocarotenoids. Caco-2 cells were grown on 6-well plastic plates until a differentiated cell monolayer was achieved. β-Apocarotenoids were prepared in Tween 40 micelles, delivered to differentiated cells in serum-free medium, and incubated at 37°C for up to 8 h. There was rapid uptake of β-apo-8'-carotenal into cells, and β-apo-8'-carotenal was largely converted to β-apo-8'-carotenoic acid and a minor metabolite that we identified as 5,6-epoxy-β-apo-8'-carotenol. There was also rapid uptake of β-apo-10'-carotenal into cells, and β-apo-10'-carotenal was converted into a major metabolite identified as 5,6-epoxy-β-apo-10'-carotenol and a minor metabolite that is likely a dihydro-β-apo-10'-carotenol. Finally, there was rapid cellular uptake of β-apo-13-carotenone, and this compound was extensively degraded. These results suggest that dietary β-apocarotenals are extensively metabolized in intestinal cells via pathways similar to the metabolism of retinal. Thus, they are likely not absorbed directly from the diet.
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durojaye2019uptakejournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Durojaye, Boluwatiwi O;Riedl, Kenneth M;Curley, Robert W;Harrison, Earl H;
Journal journal of lipid research
Year 2019
DOI
10.1194/jlr.M093161
URL
Keywords
vitamin a cell uptake β-carotene absorption intestine carotenoid metabolism dietary lipids nutrition/lipids

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