Structural basis of the mechanism of β-methyl epimerization by enzyme MarH.

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ID: 64709
2019
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Abstract
Diverse derivatives of amino acids with different steric configurations are important biosynthetic building blocks. In biology, epimerization is an important way to generate steric diversity. MarH catalyzes the epimerization of the β-position of (3R)-β-methyl-indolepyruvate (MeInPy), forming (3S)-β-MeInPy. Both compounds are derivatives of l-tryptophan (l-Trp) and are important precursors of bioactive natural products. Here, we report the crystal structures of MarH and the NMR structure of its complex with l-Trp, an analogue of its native substrate, (3R)-β-MeInPy. Structural analysis and mutagenesis studies indicated that His25 acts as a base to remove Hβ and generate a planar carbanion intermediate, which is then putatively reprotonated on the opposite face by a water molecule to form (3S)-β-MeInPy in a stereospecific manner. The details of β-site isomerization at the atomic level provide deeper insights into the epimerization mechanism of MarH and will facilitate further enzyme design to extend the substrate scope.
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liu2019structuralorganic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liu, Bin;Hou, Yan;Wang, Xiaozheng;Ma, Xiaofang;Fang, Shiqi;Huang, Tao;Chen, Yanli;Bai, Zhiqiang;Lin, Shuangjun;Zhang, Rundong;Hu, Kaifeng;
Journal Organic & biomolecular chemistry
Year 2019
DOI
10.1039/c9ob01996k
URL
Keywords
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