Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors.

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ID: 64252
2016
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Abstract
A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimation of ALP in saliva and (3) chlorpyrifos control in commercial preparations. The single-use kits developed are low cost, easy and fast to manufacture and are stable for at least 20 days at -20°C, so the zein film can preserve and deliver both the enzyme and substrates.
Reference Key
jornetmartnez2016zeinbiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jornet-Martínez, N;Campíns-Falcó, P;Hall, E A H;
Journal Biosensors & bioelectronics
Year 2016
DOI
S0956-5663(16)30548-6
URL
Keywords
biomaterial alkaline phosphatase pesticide saliva zein biosensors

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