Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors.

Clicks: 409
ID: 64252
2016
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Abstract
A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimation of ALP in saliva and (3) chlorpyrifos control in commercial preparations. The single-use kits developed are low cost, easy and fast to manufacture and are stable for at least 20 days at -20°C, so the zein film can preserve and deliver both the enzyme and substrates.
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jornetmartnez2016zeinbiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Jornet-Martínez, N;Campíns-Falcó, P;Hall, E A H;
Journal Biosensors & bioelectronics
Year 2016
DOI
S0956-5663(16)30548-6
URL
Keywords
biomaterial alkaline phosphatase pesticide saliva zein biosensors

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