Seed-specific expression and analysis of recombinant anti-HER2 single-chain variable fragment (scFv-Fc) in Arabidopsis thaliana.

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ID: 64247
2017
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Abstract
Antibodies to human epidermal growth factor receptor 2 (HER2) are a key element of breast cancer therapy; however, they are expensive to produce and their availability is limited. A seed-specific expression system can be used to produce recombinant proteins. We report a seed-specific expression system for the manufacture of anti-HER2 ScFv-Fc in Arabidopsis thaliana, driven by the Phaseolus vulgaris β-phaseolin promoter. Recombinant anti-HER2 ScFv-Fc was successfully and specifically expressed in seeds, and identified by protein analysis. The highest protein accumulation level, with a maximum of 1.1% of total soluble protein, was observed in mature seeds. We also demonstrated the anti-tumor potency of the plant-derived antibody against SK-BR-3 cells. These results suggest that seed-expression systems could contribute to the manufacture of commercial antibodies such as anti-HER2 ScFv-Fc.
Reference Key
dong2017seedspecificprotein Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Dong, Yuanyuan;Li, Jian;Yao, Na;Wang, Dezhong;Liu, Xiuming;Wang, Nan;Li, Xiaowei;Wang, Fawei;Li, Haiyan;Jiang, Chao;
Journal Protein expression and purification
Year 2017
DOI
S1046-5928(16)30220-0
URL
Keywords
recombinant protein adcc activity anti-her2 scfv transgenic plants

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