Genetic Logic Gates Enable Patterning of Amyloid Nanofibers.

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ID: 61186
2019
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Abstract
Distinct spatial patterning of naturally produced materials is observed in many cellular structures and even among communities of microorganisms. Reoccurrence of spatially organized materials in all branches of life is clear proof that organization is beneficial for survival. Indeed, organisms can trick the evolutionary process by using organized materials in ways that can help the organism to avoid unexpected conditions. To expand the toolbox for synthesizing patterned living materials, Boolean type "AND" and "OR" control of curli fibers expression is demonstrated using recombinases. Logic gates are designed to activate the production of curli fibers. The gates can be used to record the presence of input molecules and give output as CsgA expression. Two different curli fibers (CsgA and CsgA-His-tag) production are then selectively activated to explore distribution of monomers upon coexpression. To keep track of the composition of fibers, CsgA-His-tag proteins are labeled with nickel-nitrilotriacetic acid (Ni-NTA-) conjugated gold nanoparticles. It is observed that an organized living material can be obtained upon inducing the coexpression of different CsgA fibers. It is foreseen that living materials with user-defined curli composition hold great potential for the development of living materials for many biomedical applications.
Reference Key
kalyoncu2019geneticadvanced Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kalyoncu, Ebuzer;Ahan, Recep Erdem;Ozcelik, Cemile Elif;Seker, Urartu Ozgur Safak;
Journal advanced materials (deerfield beach, fla)
Year 2019
DOI
10.1002/adma.201902888
URL
Keywords
amyloid fibers biofilm patterning genetic circuits genetic logic gates recombinase

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