CRISPR-Cas9 system: A new-fangled dawn in gene editing.

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ID: 61045
2019
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Abstract
Till date, only three techniques namely Zinc Finger Nuclease (ZFN), Transcription-Activator Like Effector Nucleases (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR-Cas9) are available for targeted genome editing. CRISPR-Cas system is very efficient, fast, easy and cheap technique for achieving knock-out gene in the cell. CRISPR-Cas9 system refurbishes the targeted genome editing approach into a more expedient and competent way, thus facilitating proficient genome editing through embattled double-strand breaks in approximately any organism and cell type. The off-target effects of CRISPR Cas system has been circumnavigated by using paired nickases. Moreover, CRISPR-Cas9 has been used effectively for numerous purposes, like knock-out of a gene, regulation of endogenous gene expression, live-cell labelling of chromosomal loci, edition of single-stranded RNA and high-throughput gene screening. The execution of the CRISPR-Cas9 system has amplified the number of accessible scientific substitutes for studying gene function, thus enabling generation of CRISPR-based disease models. Even though many mechanistic questions are left behind to be answered and the system is not yet fool-proof i.e., a number of challenges are yet to be addressed, the employment of CRISPR-Cas9-based genome engineering technologies will increase our understanding to disease processes and their treatment in the near future. In this review we have discussed the history of CRISPR-Cas9, its mechanism for genome editing and its application in animal, plant and protozoan parasites. Additionally, the pros and cons of CRISPR-Cas9 and its potential in therapeutic application have also been detailed here.
Reference Key
gupta2019crisprcas9life Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Gupta, Darshana;Bhattacharjee, Oindrila;Mandal, Drishti;Sen, Madhab Kumar;Dey, Dhritiman;Dasgupta, Adhiraj;Kazi, Tawsif Ahmed;Gupta, Rahul;Sinharoy, Senjuti;Acharya, Krishnendu;Chattopadhyay, Dhrubajyoti;Ravichandiran, V;Roy, Syamal;Ghosh, Dipanjan;
Journal Life sciences
Year 2019
DOI
S0024-3205(19)30562-4
URL
Keywords
crispr-cas9 genome editing knock in knock out

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