Synthesis of carbon quantum dots-doped dummy molecularly imprinted polymer monolithic column for selective enrichment and analysis of aflatoxin B in peanut.

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ID: 60915
2018
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Abstract
In this study, a novel method was proposed to sensitively determine aflatoxin B (AFB) in peanut sample by using a carbon quantum dots-coated dummy molecularly imprinted polymer (CDs-DMIP) monolithic column for pretreatment coupled with high performance liquid chromatography-fluorescence detection (HPLC-FLD). The CDs-DMIP monolithic column was prepared by in-situ polymerization in a water bath using 5,7-dimethoxycoumarin as dummy template molecule. The CDs-DMIP monolithic column was applied to determine AFB by HPLC-FLD. Satisfactory linearity was obtained over 0.5-2000ngmL, with a high correlation coefficient of 0.9999. The recoveries of AFB in peanut sample ranged from 79.5% to 91.2%, and the intraday and interday relative standard deviation ranged from 1.2% to 4.9%. Limit of detection (S/N=3) and limit of quantitation (S/N=10) were 0.118ngmL and 0.393ngmL, respectively. Under the optimized conditions, the enrichment factor was over 71-fold. AFB in peanut sample and even some other samples could be sensitively determined by CDs-DMIP-HPLC-FLD method.
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liang2018synthesisjournal Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liang, Guohuan;Zhai, Haiyun;Huang, Lu;Tan, Xuecai;Zhou, Qing;Yu, Xiao;Lin, Haidan;
Journal Journal of pharmaceutical and biomedical analysis
Year 2018
DOI
S0731-7085(17)31931-3
URL
Keywords
High performance liquid chromatography carbon quantum dots aflatoxin b(1) molecularly imprinted monolithic columns

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