met1 DNA Methyltransferase Controls TERT Gene Expression: A New Insight to The Role of Telomerase in Development.

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ID: 60779
2020
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Abstract
DNA methylation systems are essential for proper embryo development. Methylation defects lead to developmental abnormalities. Furthermore, changes in telomerase gene expression can affect stability of chromosomes and produces abnormal growth. Therefore, defects in both methylation and telomerase gene expression can lead to developmental abnormalities. We hypothesized that mutation in the methylation systems may induce developmental abnormalities through changing telomerase gene expression.In this experimental study, we used (At) as a developmental model. DNA was extracted from seedlings leaves. The grown plants were screened using polymerase chain reaction (PCR) reactions. Total RNA was isolated from the mature leaves, stems and flowers of wild type and mutants. For gene expression analysis, cDNA was synthesized and then quantitative reverse transcription PCR (qRT-PCR) was performed.Telomerase gene expression level in homozygous mutant plants showed ~14 fold increase compared to normal plants. Furthermore, expression in heterozygous was~ 2 fold higher than the wild type plants.Our results suggested that is a methyltransferase-regulated gene which may be involved in developmental abnormities causing by mutation in methyltransferase system.
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zangi2020met1cell Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zangi, Maryam;Bagherieh Najjar, Mohammad Bagher;Golalipour, Masoud;Aghdasi, Mahnaz;
Journal cell journal
Year 2020
DOI
10.22074/cellj.2020.6290
URL
Keywords
telomerase developmental abnormalities met1

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