Rapid detection of aflatoxin B by dummy template molecularly imprinted polymer capped CdTe quantum dots.

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ID: 59965
2019
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Abstract
A novel and sensitive fluorescent sensor was synthesized for the rapid and specific recognition of aflatoxin B (AFB1) by our combining molecular imprinting techniques with quantum dot technology. Molecularly imprinted polymers coated CdTe quantum dots (MIP@CdTe QDs) were prepared through the Stöber method with 5,7-dimethoxycoumarin as a dummy template. 3-Aminopropyltriethoxysilane was selected as the functional monomer, and tetraethyl orthosilicate was used as the cross-linking agent. The best molar ratio of 5,7-dimethoxycoumarin to functional monomer to cross-linker was 4:20:15. The MIP@CdTe QD composites were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence spectroscopy. Under the optimum conditions, the relative fluorescence intensity of the MIP@CdTe QDs showed adequate linearity with AFB1 concentration over the range from 80 to 400 ng/g. The detection limit is 4 ng/g, according to 3s/K. Finally, the method was successfully applied to the quantitative determination of AFB1 in real samples. The spike recoveries at different spiking levels ranged from 99.20% to 101.78%, which were consistent with those measured by ultrahigh-performance liquid chromatography-mass spectrometry. The method developed for AFB1 detection lays the foundation for rapid detection of trace amounts of other exogenous harmful substances in a complicated matrix.
Reference Key
guo2019rapidanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pengqi Guo, Wu Yang, Hao Hu, Yitao Wang, Peng Li
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Journal Analytical and bioanalytical chemistry
Year 2019
DOI
10.1007/s00216-019-01708-2
URL
Keywords
aflatoxin b1 cdte quantum dots dummy template molecularly imprinted polymers fluorescent probe

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