Metal-Mediated Gold Nanospheres Assembled for Dark-Field Microscopy Imaging Scatterometry.

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ID: 59956
2019
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Abstract
Developing rapid, sensitive and intelligent optical probes is important for the growing need of microscope imaging analysis. Herein, we proposed a new strategy to assemble plasmonic nanoprobes in situ for dark-field microscopy imaging scatterometry by making use of the formation, disruption, and re-formation of cytosine-Ag-cytosine (CAgC) bonds. The CAgC bond was formed at first through Ag-mediated base pairing between C-contained aptamer and its C-mismatched complementary DNA. Owing to the subsequent binding of target with the aptamer, the CAgC structure was disrupted, leading Ag to be quantitatively released. The released Ag ions can make the CAgC bonds formed again between the C-contained sequence that modified gold nanospheres (AuNSs), and AuNS clusters thus formed in situ, which have strong plasmonic scattering signals owing to the coupling of localized surface plasmon resonance (LSPR). Therefore, the plasmonic scattering signals enhanced following the off-on mode under the dark field microscope from the 'zero' background to on. As a concept of proof, sensitive detections for Aflatoxin B1 (AFB) in foods, carcinoembryonic antigen (CEA) in blood serum, and Ricin B in artificial sample, was successfully made by using of the in situ formed AuNS clusters, demonstrating that the newly developed metal-mediated strategy for assembling nanoprobes are universal.
Reference Key
xie2019metalmediatedtalanta Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Xie, Yi Fen;Liu, Meng Li;Lv, Wen Yi;Li, Xue;Gao, Peng Fei;Li, Yuan Fang;Huang, Cheng Zhi;
Journal Talanta
Year 2019
DOI
S0039-9140(19)30389-3
URL
Keywords
chemometrics aflatoxin b(1) rapid determination soybean oil terahertz spectroscopy

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