A simple and efficient method for potential point-of-care diagnosis of human papillomavirus genotypes: combination of isothermal recombinase polymerase amplification with lateral flow dipstick and reverse dot blot.

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ID: 58915
2019
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Ranked #14 of 90 articles by views in Analytical and bioanalytical chemistry

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Resumo
Cervical cancer is the second most common cancer in the world's woman population with a high incidence in developing countries where diagnostic conditions for the cancer are poor. The main culprit causing the cancer is the human papillomavirus (HPV). HPV is divided into three major groups, i.e., high-risk (HR) group, probable high-risk (pHR) group, and low-risk (LR) group according to their potential of causing cervical cancer. Therefore, developing a sensitive, reliable, and cost-effective point-of-care diagnostic method for the virus genotypes in developing countries even worldwide is of high importance for the cancer prevention and control strategies. Here we present a combined method of isothermal recombinase polymerase amplification (RPA), lateral flow dipstick (LFD), and reverse dot blot (RDB), in quick point-of-care identification of HPV genotypes. The combined method is highly specific to HPV when the conserved L1 genes are used as targeted genes for amplification. The method can be used in identification of HPV genotypes at point-of-care within 1 h with a sensitivity of low to 100 fg of the virus genomic DNA. We have demonstrated that it is an excellent diagnostic point-of-care assay in monitoring the disease without time-consuming and expensive procedures and devices.
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ma2019aanalytical Use esta chave para citacao automatica no manuscrito ao usar SciMatic Gerenciador de Manuscritos ou Gerenciador de Teses
Autores Biao Ma, Jiehong Fang, Wei Lin, Xiaoping Yu, Chuanxin Sun, Mingzhou Zhang
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Periodico Analytical and bioanalytical chemistry
Ano 2019
DOI
10.1007/s00216-019-02113-5
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Palavras-chave
human papillomavirus (hpv) visual detection point-of-care testing (poct) lateral flow dipstick (lfd) recombinase polymerase amplification (rpa) reverse dot blot (rdb)

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