Biochemical Characterization of the Candidate ACC-Deaminases and Virulence Testing of Knockout Mutant Strains.

Clicks: 379
ID: 57236
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #23 of 187 articles by views in Frontiers in plant science

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 187 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
is a plant pathogenic fungus which is able to infect wheat and other economically important cereal crop species. The role of ethylene in the interaction with host plants is unclear and controversial. We have analyzed the inventory of genes with a putative function in ethylene production or degradation of the ethylene precursor 1-aminocyclopropane carboxylic acid (ACC). , in contrast to other species, does not contain a candidate gene encoding ethylene-forming enzyme. Three genes with similarity to ACC synthases exist; heterologous expression of these did not reveal enzymatic activity. The genome contains in addition two ACC deaminase candidate genes. We have expressed both genes in and characterized the enzymatic properties of the affinity-purified products. One of the proteins had indeed ACC deaminase activity, with kinetic properties similar to ethylene-stress reducing enzymes of plant growth promoting bacteria. The other candidate was inactive with ACC but turned out to be a d-cysteine desulfhydrase. Since it had been reported that ethylene insensitivity in transgenic wheat increased resistance and reduced the content of the mycotoxin deoxynivalenol (DON) in infected wheat, we generated single and double knockout mutants of both genes in the strain PH-1. No statistically significant effect of the gene disruptions on fungal spread or mycotoxin content was detected, indicating that the ability of the fungus to manipulate the production of the gaseous plant hormones ethylene and HS is dispensable for full virulence.
Reference Key
svoboda2019biochemicalfrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Svoboda, Thomas;Parich, Alexandra;Güldener, Ulrich;Schöfbeck, Denise;Twaruschek, Krisztian;Václavíková, Marta;Hellinger, Roland;Wiesenberger, Gerlinde;Schuhmacher, Rainer;Adam, Gerhard;
Journal Frontiers in plant science
Year 2019
DOI
10.3389/fpls.2019.01072
URL
Keywords
fusarium graminearum acc deaminase acc (1-aminocyclopropane-1-carboxylic acid) acc synthase ketobutyrate

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.