Tubulin-Dependent Transport of Connexin-36 Potentiates the Size and Strength of Electrical Synapses.

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ID: 55843
2019
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Abstract
Connexin-36 (Cx36) electrical synapses strengthen transmission in a calcium/calmodulin (CaM)/calmodulin-dependent kinase II (CaMKII)-dependent manner similar to a mechanism whereby the N-methyl-D-aspartate (NMDA) receptor subunit NR2B facilitates chemical transmission. Since NR2B-microtubule interactions recruit receptors to the cell membrane during plasticity, we hypothesized an analogous modality for Cx36. We determined that Cx36 binding to tubulin at the carboxy-terminal domain was distinct from Cx43 and NR2B by binding a motif overlapping with the CaM and CaMKII binding motifs. Dual patch-clamp recordings demonstrated that pharmacological interference of the cytoskeleton and deleting the binding motif at the Cx36 carboxyl-terminal (CT) reversibly abolished Cx36 plasticity. Mechanistic details of trafficking to the gap-junction plaque (GJP) were probed pharmacologically and through mutational analysis, all of which affected GJP size and formation between cell pairs. Lys279, Ile280, and Lys281 positions were particularly critical. This study demonstrates that tubulin-dependent transport of Cx36 potentiates synaptic strength by delivering channels to GJPs, reinforcing the role of protein transport at chemical and electrical synapses to fine-tune communication between neurons.
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brown2019tubulindependentcells Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Brown, Cherie A;Del Corsso, Cristiane;Zoidl, Christiane;Donaldson, Logan W;Spray, David C;Zoidl, Georg;
Journal Cells
Year 2019
DOI
E1146
URL
Keywords
transport gap junction cytoskeleton connexin-36 (cx36) electrical plasticity tubulin and microtubules

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