Ion-Ion Proton Transfer and Parallel Ion Parking for the Analysis of Mixtures of Intact Proteins on a Modified Orbitrap Mass Analyzer.

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2019
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Abstract
We have enabled parallel ion parking on a modified Orbitrap Elite™ as a way to control ion-ion proton transfer reactions via selective activation of a range of ions. The result is the concentration of the majority of ion current from multiple charge states of each precursor proteoform into a single charge state, maximizing signal intensity and increasing effective sensitivity compared to conventional MS1 spectra. These techniques were applied in an on-line HPLC, data-dependent MS/MS analysis of intact E. coli ribosomal proteins with HCD fragmentation. With one injection, all but two ribosomal proteins were selected for fragmentation and subsequently identified. The techniques described facilitate rapid identification of intact proteins in complex mixtures and an enhanced ability to observe proteins of low abundance.
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Authors Ugrin, Scott A;English, A Michelle;Syka, John E P;Bai, Dina L;Anderson, Lissa C;Shabanowitz, Jeffrey;Hunt, Donald F;
Journal journal of the american society for mass spectrometry
Year 2019
DOI
10.1007/s13361-019-02290-8
URL
Keywords
intact protein ion-ion proton transfer reactions parallel ion parking quadrupole linear ion trap reagent activation top-down

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