YtrA, a GntR-Family Transcription Factor, Represses Two Genetic Loci Encoding Membrane Proteins in .

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ID: 55217
2019
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Abstract
In bacteria, the GntR family is a widespread family of transcription factors responsible for the regulation of a myriad of biological processes. In contrast, despite their occurrence in archaea only a little information is available on the function of GntR-like transcription factors in this domain of life. The thermoacidophilic crenarchaeon harbors a GntR-like regulator belonging to the YtrA subfamily, encoded as the first gene in an operon with a second gene encoding a putative membrane protein. Here, we present a detailed characterization of this regulator, named YtrA, with a focus on regulon determination and mechanistic analysis with regards to DNA binding. Genome-wide chromatin immunoprecipitation and transcriptome experiments, the latter employing a overexpression strain, demonstrate that the regulator acts as a repressor on a very restricted regulon, consisting of only two targets including the operon encoding its own gene and a distinct genetic locus encoding another putative membrane protein. For both targets, a conserved 14-bp semi-palindromic binding motif was delineated that covers the transcriptional start site and that is surrounded by additional half-site motifs. The crystallographic structure of YtrA was determined, revealing a compact dimeric structure in which the DNA-binding motifs are oriented ideally to enable a specific high-affinity interaction with the core binding motif. This study provides new insights into the functioning of a YtrA-like regulator in the archaeal domain of life.
Reference Key
lemmens2019ytrafrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liesbeth Lemmens, Laurentijn Tilleman, Ezra De Koning, Karin Valegård, Ann-Christin Lindås, Filip Van Nieuwerburgh, Dominique Maes, Eveline Peeters
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Journal Frontiers in microbiology
Year 2019
DOI
10.3389/fmicb.2019.02084
URL
Keywords
gntr sulfolobus ytra archaea membrane protein transcription regulation

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