Time-resolved crystallography reveals allosteric communication aligned with molecular breathing.

Clicks: 442
ID: 54626
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #7 of 34 articles by views in science (new york, ny)

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
A comprehensive understanding of protein function demands correlating structure and dynamic changes. Using time-resolved serial synchrotron crystallography, we visualized half-of-the-sites reactivity and correlated molecular-breathing motions in the enzyme fluoroacetate dehalogenase. Eighteen time points from 30 milliseconds to 30 seconds cover four turnover cycles of the irreversible reaction. They reveal sequential substrate binding, covalent-intermediate formation, setup of a hydrolytic water molecule, and product release. Small structural changes of the protein mold and variations in the number and placement of water molecules accompany the various chemical steps of catalysis. Triggered by enzyme-ligand interactions, these repetitive changes in the protein framework's dynamics and entropy constitute crucial components of the catalytic machinery.
Reference Key
mehrabi2019timeresolvedscience Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mehrabi, Pedram;Schulz, Eike C;Dsouza, Raison;Müller-Werkmeister, Henrike M;Tellkamp, Friedjof;Miller, R J Dwayne;Pai, Emil F;
Journal science (new york, ny)
Year 2019
DOI
10.1126/science.aaw9904
URL
Keywords
computational fluid dynamics mechanical engineering hydrodynamics fink's guide vane fluid mechanics hydrodynamic instability reversible francis turbine unsteady cfd

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.