Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.

Clicks: 475
ID: 54612
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #4 of 61 articles by views in Nature Methods

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The limited per-pixel bandwidth of most microscopy methods requires compromises between field of view, sampling density and imaging speed. This limitation constrains studies involving complex motion or fast cellular signaling, and presents a major bottleneck for high-throughput structural imaging. Here, we combine high-speed intensified camera technology with a versatile, reconfigurable and dramatically improved Swept, Confocally Aligned Planar Excitation (SCAPE) microscope design that can achieve high-resolution volumetric imaging at over 300 volumes per second and over 1.2 GHz pixel rates. We demonstrate near-isotropic sampling in freely moving Caenorhabditis elegans, and analyze real-time blood flow and calcium dynamics in the beating zebrafish heart. The same system also permits high-throughput structural imaging of mounted, intact, cleared and expanded samples. SCAPE 2.0's significantly lower photodamage compared to point-scanning techniques is also confirmed. Our results demonstrate that SCAPE 2.0 is a powerful, yet accessible imaging platform for myriad emerging high-speed dynamic and high-throughput volumetric microscopy applications.
Reference Key
voleti2019realtimenature Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Voleti, Venkatakaushik;Patel, Kripa B;Li, Wenze;Perez Campos, Citlali;Bharadwaj, Srinidhi;Yu, Hang;Ford, Caitlin;Casper, Malte J;Yan, Richard Wenwei;Liang, Wenxuan;Wen, Chentao;Kimura, Koutarou D;Targoff, Kimara L;Hillman, Elizabeth M C;
Journal Nature Methods
Year 2019
DOI
10.1038/s41592-019-0579-4
URL
Keywords
cell cycle aurka fret fluorescence microscopy non-mitotic roles structural data

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.