Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass-spectrometry.

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ID: 52827
2019
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Abstract
Aspergillus flavus is a major cause of severe non-invasive fungal infections in the Middle Eastern countries. However, it is difficult to distinguish A. flavus from A. oryzae.To assess the potential of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in discriminating between A. flavus and A. oryzae and compare it with β-tubulin gene sequencing.We used the Bruker Daltonik MALDI-TOF MS system to analyze 200 clinical and environmental A. flavus isolates and one A. pseudonomius and one A. alliaceus (Aspergillus section Flavi) isolates a priori identified as such by sequencing of the β-tubulin gene.All 200 A. flavus isolates were identified at the genus level and 176 (88%) at the species levels by MALDI-TOF MS based on the spectral log-scores (≥ 2.0 and 1.7-1.99, respectively); among them, only 18 (10.2%) were confirmed as A. flavus, whereas 35 (19.9%) were identified as A. oryzae and 123 (69.9%) as A. flavus/A. oryzae. Aspergillus pseudonomius and A. alliaceus were misidentified as A. flavus and A. parasiticus with log-score values of 1.39 and 1.09, respectively.The results indicate that the commercially available Bruker Daltonik MALDI-TOF MS score database cannot separate A. flavus and A. oryzae species. We also showed that establishment of an in-house library is a useful tool to discriminate closely-related Aspergillus species, including A. flavus and A. oryzae.
Reference Key
hedayati2019discriminationmycoses Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hedayati, Mohammad T;Taghizadeh-Armaki, Mojtaba;Zarrinfar, Hossein;Hoseinnejad, Akbar;Ansari, Saham;Abastabar, Mahdi;Er, Halil;Özhak, Betil;Öğünç, Dilara;Ilkit, Macit;Seyedmousavi, Seyedmojtaba;
Journal Mycoses
Year 2019
DOI
10.1111/myc.13010
URL
Keywords
identification aspergillus flavus aspergillus oryzae maldi-tof ms β-tubulin

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