A novel adaptive fluorescent probe for cell labelling.

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ID: 51602
2019
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Ranked #18 of 133 articles by views in Bioorganic chemistry

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Abstract
In this study we describe the synthesis and characterisation of a new hydrazone-based fluorescent compound that is able to selectively label the endoplasmic reticulum (ER) in yeast and mammalian living cells. The fluorescence properties of the compound depended on the DMSO/water ratio and on the pH. NMR experiments allowed determination of the conformation adopted in various environments. Apart from the convenient synthetic procedure, our compound displays low cell toxicity and blue emission compatible with filters routinely used in fluorescence microscopy.
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coman2019abioorganic Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Coman, Anca G;Paun, Anca;Popescu, Codruta C;Hădade, Niculina D;Hanganu, Anamaria;Chiritoiu, Gabriela;Farcasanu, Ileana C;Matache, Mihaela;
Journal Bioorganic chemistry
Year 2019
DOI
S0045-2068(19)30812-0
URL
Keywords
synthesis fluorescence endoplasmic reticulum cell labelling n-acylhydrazone

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