A method for generating virus-free cassava plants to combat viral disease epidemics in Africa.

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ID: 50979
2019
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Abstract
Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases.
Reference Key
maruthi2019aphysiological Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Maruthi, M N;Whitfield, E Charles;Otti, Gerald;Tumwegamire, Silver;Kanju, Edward;Legg, James P;Mkamilo, Geoffrey;Kawuki, Robert;Benesi, Ibrahim;Zacarias, Anabela;Munga, Therezia;Mwatuni, Francis;Mbugua, Edward;
Journal physiological and molecular plant pathology
Year 2019
DOI
10.1016/j.pmpp.2018.09.002
URL
Keywords
cassava mosaic disease acmv, african cassava mosaic virus cbsd, cassava brown streak disease cbsv, cassava brown streak virus cmd, cassava mosaic disease ctab, cetyl trimethylammonium bromide cassava brown streak disease eacmv, east african cassava mosaic virus eacmv-ug, east african cassava mosaic virus-uganda lfc, laminar flow cabinet ms, murashige and skoog manihot esculenta ppm, plant preservation mixture rh, relative humidity sdw, sterilised deionised water ucbsv, ugandan cassava brown streak virus uic, unique identifying code virus diagnosis virus indexing

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