Molecular analysis of V617F mutation in Janus kinase 2 gene of breast cancer patients.

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ID: 49092
2019
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Abstract
Breast cancer is a multifactorial disease with the highest frequency in females. Genetic and environmental factors can cause mutation in several genes like tyrosine kinase, JAK2 gene which may initiate cancer. Molecular analysis of mutations in the gene along with determination of environmental, clinical and haematological risk factors associated with breast cancer patients is need of hour to improve patient's healthcare. Somatic JAK2 valine-to-phenylalanine (617 codon) mutation is one of the widely prevalent mutations.Blood was collected from seventy breast cancer patients after their consent. The questionnaire included risk factors, age group, locality, number of children, tumor type, family history, time of initial diagnosis, no of cycles/month, water conditions and exposure to radiations. Molecular analysis were carried out from genomic DNA using Sanger sequencing and allele-specific PCR to check the V617F point mutation.The breast cancer risk factors includes unfiltered water (68.57%), urban (58.57%), menopause (55.71%), family history of cancer (18.57%), tumor grades (II, 37.14% and III, 35.71%), consanguineous marriages (44.28%) and having more than 3-4 children (45.71%). Prevalence of breast cancer was higher after the age of 35 and maximum at 35-50. In allele-specific PCR of 70 patients, 25 patients were wild type (229 bp), 25 patients were with partially deleted gene (200 bp), and 20 patient had shown no or less than 40 bp size fragments. In Sanger's sequencing of 70 BC cases, 18% were found to be positive for V617F point mutation, including 6 homozygous (T/T) and 7 heterozygous (G/T) mutations at nucleotide position 1849 in exon 14 of the gene.Environmental and clinical risk factors were associated with breast cancer which can be overcome by improving awareness of associated risks, health facilities and reducing stress.
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karim2019molecularsaudi Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Karim, Sajjad;Malik, Imran Riaz;Nazeer, Quratulain;Zaheer, Ahmad;Farooq, Muhammad;Mahmood, Nasir;Malik, Arif;Asif, Muhammad;Mehmood, Asim;Khan, Abdul Rehman;Jabbar, Abdul;Arshad, Muhammad;Yousafi, Qudsia;Hussain, Abrar;Mirza, Zeenat;Iqbal, Muhammad Atif;Rasool, Mahmood;
Journal saudi journal of biological sciences
Year 2019
DOI
10.1016/j.sjbs.2019.08.002
URL
Keywords
Breast cancer allele-specific pcr jak2 sanger’s sequencing v617f mutation

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