Metaproteomics: Sample Preparation and Methodological Considerations.

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ID: 48843
2019
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Abstract
Meta-omic techniques have progressed rapidly in the past decade and are frequently used in microbial ecology to study microorganisms in their natural ecosystems independent from culture restrictions. Metaproteomics, in combination with metagenomics, enables quantitative assessment of expressed proteins and pathways from individual members of the consortium. Together, metaproteomics and metagenomics can provide a detailed understanding of which organisms occupy specific metabolic niches, how they interact, and how they utilize nutrients, and these insights can be obtained directly from environmental samples. Here, we outline key aspects of sample preparation, database generation, and other methodological considerations that are required for successful quantitative metaproteomic analyses and we describe case studies on the integration with metagenomics for enhanced functional output.
Reference Key
kunath2019metaproteomicsadvances Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kunath, Benoit J;Minniti, Giusi;Skaugen, Morten;Hagen, Live H;Vaaje-Kolstad, Gustav;Eijsink, Vincent G H;Pope, Phil B;Arntzen, Magnus Ø;
Journal advances in experimental medicine and biology
Year 2019
DOI
10.1007/978-3-030-12298-0_8
URL
Keywords
metagenomics metaproteomics microbial communities assembly functional analysis binning

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