Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions.

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ID: 47866
2018
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Abstract
Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region. Back correlation of the proteins identified by spatially-resolved proteomics at a given tissue localization with the MALDI MS imaging data, was then performed. As an example, mapping of the distribution of the matrix metallopeptidase 3-cleaved C-terminal fragment of α-synuclein (aa 95-140) identified its specific distribution along the hippocampal dentate gyrus. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
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delcourt2018spatiallyresolvedmolecular Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Delcourt, Vivian;Franck, Julien;Quanico, Jusal;Gimeno, Jean-Pascal;Wisztorski, Maxence;Raffo-Romero, Antonella;Kobeissy, Firas;Roucou, Xavier;Salzet, Michel;Fournier, Isabelle;
Journal molecular & cellular proteomics : mcp
Year 2018
DOI
10.1074/mcp.M116.065755
URL
Keywords
Invasion Migration adhesion deguelin nci-h292 cells nf-b

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