Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions.

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2018
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Abstract
Tissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region. Back correlation of the proteins identified by spatially-resolved proteomics at a given tissue localization with the MALDI MS imaging data, was then performed. As an example, mapping of the distribution of the matrix metallopeptidase 3-cleaved C-terminal fragment of α-synuclein (aa 95-140) identified its specific distribution along the hippocampal dentate gyrus. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
Reference Key
delcourt2018spatiallyresolvedmolecular Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Delcourt, Vivian;Franck, Julien;Quanico, Jusal;Gimeno, Jean-Pascal;Wisztorski, Maxence;Raffo-Romero, Antonella;Kobeissy, Firas;Roucou, Xavier;Salzet, Michel;Fournier, Isabelle;
Journal molecular & cellular proteomics : mcp
Year 2018
DOI
10.1074/mcp.M116.065755
URL
Keywords
Invasion Migration adhesion deguelin nci-h292 cells nf-b

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