sp. nov., an actinobacterium isolated from soil.

A Gram-positive, aerobic, non-spore-forming, non-pigmented and non-motile actinobacterial strain was isolated from a soil sample collected in Guangzhou, China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 130 is most closely related to the type strain NBRC 105384, with a sequence similarity of 97.69 %. The isolate was distinguished from this phylogenetically related type strain by DNA-DNA hybridization (33.3 %), digital DNA-DNA hybridization (21.2 %), average nucleotide identity (75.7 %) and by a range of physiological and biochemical characteristics. Strain 130 contained MK-8(H) and MK-7 as the major menaquinones, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol as the main polar lipids, and iso-C, Cω8, anteiso-C, Cω9, C and iso-C as the major cellular fatty acids. The novel strain grew at 20-36 °C, at pH 6.0-8.0 and in the presence of 0-6 % (w/v) NaCl. The genomic DNA G+C content was 72.9 mol%. The genome contained 4817 putative protein-coding sequences, and 45 tRNA and three rRNA genes. Phylogenomic analysis confirmed that strain 130 belongs to the genus and distinguished it from recognized species with available genomes. Based on these polyphasic taxonomic data, strain 130 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is 130 (=CICC 24668=JCM 33269).