Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63

Clicks: 446
ID: 47550
2019
Free PDF
Article Quality & Performance Metrics
Overall Quality Improving Quality
0.0 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment
Not analyzed
Abstract
Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of the assay to significantly distinguish HCoV-NL63 from HCoV-229E (U = 27.50, p < 0.001) and HCoV-OC43 (U = 55.50, p < 0.001) in coronavirus-characterized sera. Evaluation of the assay with collected human samples showed no significant difference in mean optical density values of immunofluorescence-classified HCoV-NL63-positive and HCoV-NL63-negative samples (F (1, 215) = 0.437, p = 0.509). All the top 5% (n = 8) most reactive human samples tested by ELISA were HCoV-NL63 positive by immunofluorescence testing. In comparison, only a proportion (84%, n = 42) of the top 25% were positive by immunofluorescence testing, indicating an increased probability of the highly ELISA reactive samples testing positive by the immunofluorescence assay. None of the top 5% most ELISA reactive livestock samples were positive for HCoV-NL63-related viruses by immunofluorescence confirmation. Ghanaian domestic livestock are not likely intermediate hosts of HCoV-NL63-related coronaviruses.
Reference Key
elduah2019developmentviruses Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors El-Duah, Philip;Meyer, Benjamin;Sylverken, Augustina;Owusu, Michael;Gottula, Lina Theresa;Yeboah, Richmond;Lamptey, Jones;Frimpong, Yaw Oppong;Burimuah, Vitus;Folitse, Raphael;Agbenyega, Olivia;Oppong, Samuel;Adu-Sarkodie, Yaw;Drosten, Christian;
Journal Viruses
Year 2019
DOI
DOI not found
URL
http://www.mdpi.com/1999-4915/11/1/43
Keywords
Microbiology Engineering (General). Civil engineering (General) Agriculture (General) Dentistry environmental sciences geography (general) neurophysiology and neuropsychology neurosciences. biological psychiatry. neuropsychiatry agriculture animal culture environmental technology. sanitary engineering

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.