Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying.

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ID: 47425
2019
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Abstract
The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18% of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals.
Reference Key
auvinen2019effectscryobiology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Auvinen, Vili-Veli;Merivaara, Arto;Kiiskinen, Jasmi;Paukkonen, Heli;Laurén, Patrick;Hakkarainen, Tiina;Koivuniemi, Raili;Sarkanen, Riina;Ylikomi, Timo;Laaksonen, Timo;Yliperttula, Marjo;
Journal cryobiology
Year 2019
DOI
S0011-2240(19)30211-1
URL
Keywords
freeze-drying 3d cell culture adipose tissue extract cell spheroids nanofibrillated cellulose

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