Dietary Supplementation of Methyl Donor L-Methionine Alters Epigenetic Modification in Type 2 Diabetes.

Clicks: 418
ID: 46892
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #5 of 35 articles by views in Molecular nutrition & food research

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The aim of the current study is to evaluate whether L-Methionine supplementation (L-Met-S) improves type 2 diabetes-induced alterations in glucose and lipid metabolism by modulating one carbon metabolism and methylation status.Diabetes was induced in male Sprague Dawley rats using high fat diet and low dose streptozotocin. At the end of study, various biochemical parameters, immunoblotting, qRT-PCR and ChIP-qPCR were performed. We provide first evidence that L-Met-S activates p-AMPK and SIRT1, very similar to 'metformin'. L-Met-S improves the altered key one-carbon metabolites in diabetic rats by modulating methionine adenosyl transferase 1A and cystathione β synthase expression. qRT-PCR shows that L-Met-S alleviates diabetes-induced increase in Forkhead transcription factor 1 expression and thereby regulating genes involved in glucose (G6pc, Pdk4, Pklr) and lipid metabolism (Fasn). Interestingly, L-Met-S inhibits the increased expression of DNMT1 and also prevents methylation of histone H3K36me2 under diabetic condition. ChIP assay shows that persistent increase in abundance of histone H3K36me2 on the promoter region of FOXO1 in diabetic rats and it is recovered by L-Met-S.We provide the first evidence that dietary supplementation of L-Met prevents diabetes-induced epigenetic alterations and regulating methionine levels can be therapeutically exploited for the treatment of metabolic diseases. This article is protected by copyright. All rights reserved.
Reference Key
navik2019dietarymolecular Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Navik, Umashanker;Sheth, Vaibhav G;Kabeer, Shaheen Wasil;Tikoo, Kulbhushan;
Journal Molecular nutrition & food research
Year 2019
DOI
10.1002/mnfr.201801401
URL
Keywords
type 2 diabetes mellitus ampk and foxo1 h3k36me2 l-methionine one-carbon metabolism

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.