Ex vivo electrochemical measurement of glutamate release during spinal cord injury.

Clicks: 411
ID: 45396
2019
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Abstract
Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.
Reference Key
nolan2019exmethodsx Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Nolan, James K;Nguyen, Tran N H;Fattah, Mara;Page, Jessica C;Shi, Riyi;Lee, Hyowon;
Journal methodsx
Year 2019
DOI
10.1016/j.mex.2019.08.008
URL
Keywords
Biosensor additive manufacturing implantable direct ink writing electrochemical glutamate sensing from resected spinal cord segment excitotoxicity rapid prototyping sci

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