Electrical Biosensing at Physiological Ionic Strength Using Graphene Field-Effect Transistor in Femtoliter Microdroplet.

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ID: 44466
2019
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Abstract
Graphene has strong potential for electrical biosensing owing to its two-dimensional nature and high carrier mobility which transduce the direct contact of a detection target with a graphene channel to a large conductivity change in a graphene field-effect transistor (G-FET). However, the measurable range from the graphene surface is highly restricted by Debye screening, whose characteristic length is less than 1 nm at physiological ionic strength. Here, we demonstrated electrical biosensing utilizing the enzymatic products of the target. We achieved quantitative measurements of a target based on the site-binding model and real-time measurement of the enzyme kinetics in femtoliter microdroplets. The combination of a G-FET and microfluidics, named a "lab-on-a-graphene-FET", detected the enzyme urease with high sensitivity in the zeptomole range in 100 mM sodium phosphate buffer. Also, the lab-on-a-graphene-FET detected the gastric cancer pathogen Helicobacter pylori captured at a distance greater than the Debye screening length from the G-FET.
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ono2019electricalnano Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ono, Takao;Kanai, Yasushi;Inoue, Koichi;Watanabe, Yohei;Nakakita, Shin-Ichi;Kawahara, Toshio;Suzuki, Yasuo;Matsumoto, Kazuhiko;
Journal Nano letters
Year 2019
DOI
10.1021/acs.nanolett.9b01335
URL
Keywords
graphene debye screening droplet enzymatic reaction

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