Protective Dps-DNA co-crystallization in stressed cells: an in vitro structural study by small-angle X-ray scattering and cryo-electron tomography.

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ID: 43027
2019
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Abstract
Under severe or prolonged stress, bacteria produce a nonspecific DNA-binding protein (Dps), which effectively protects DNA against damaging agents both in vitro and in vivo by forming intracellular biocrystals. The phenomenon of protective crystallization of DNA in living cells has been intensively investigated during the last two decades; however, the results of studies are somewhat contradictory, and up to now, there has been no direct determination of a Dps-DNA crystal structure. Here, we report the in vitro analysis of the vital process of Dps-DNA co-crystallization using two complementary structural methods: synchrotron small-angle X-ray scattering in solution and cryo-electron tomography. Importantly, for the first time, the DNA in the co-crystals was visualized, and the lattice parameters of the crystalline Dps-DNA complex were determined.
Reference Key
dadinova2019protectivefebs Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Dadinova, Liubov A;Chesnokov, Yurii M;Kamyshinsky, Roman A;Orlov, Ivan A;Petoukhov, Maxim V;Mozhaev, Andrey A;Soshinskaya, Ekaterina Yu;Lazarev, Vassili N;Manuvera, Valentin A;Orekhov, Anton S;Vasiliev, Alexander L;Shtykova, Eleonora V;
Journal FEBS Letters
Year 2019
DOI
10.1002/1873-3468.13439
URL
Keywords
dna dps biocrystallization cryo-electron tomography small-angle x-ray scattering

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