Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking.

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ID: 43014
2019
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Ranked #2 of 22 articles by views in Luminescence : the journal of biological and chemical luminescence

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Abstract
The over-use of antibiotics has caused a number of problems such as contamination of antibiotic residues and virus resistance, and therefore has attracted global attention. In this study, spectroscopic techniques and molecular docking were employed to predict conformational changes and binding interaction between two cephalosporins (cefaclor and cefixime) and calf thymus DNA (ctDNA). Fluorescence and UV-vis spectra suggested that static quenching was predominant and cephalosporin bound to the groove region of ctDNA. Binding parameters calculated by the Stern-Volmer and Scatchard equations showed that cephalosporin bound to ctDNA with a binding affinity in the order of 10  L mol . Thermodynamic parameters further indicated that the reaction was a spontaneous process driven by enthalpy and entropy, and that the main binding force was an electrostatic force. The effects of iodide, denaturant, thermal denaturation and pH on a cephalosporin-Hoechst-DNA complex were also studied, and the results confirmed that cephalosporin bound to the groove area of DNA. Finally, these results were further confirmed by molecular docking and electrochemical studies.
Reference Key
huang2019comparativeluminescence Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Huang, Sheng-Chao;Cheng, Li-Yang;Yang, Jing;Hu, Yan-Jun;
Journal Luminescence : the journal of biological and chemical luminescence
Year 2019
DOI
10.1002/bio.3696
URL
Keywords
spectroscopy molecular docking cefaclor calf thymus dna cefixime

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