In vitro and in vivo inhibition of Hass avocado polyphenol oxidase enzymatic browning by paeonol, β-cyclodextrin, and paeonol:β-cyclodextrin inclusion complex.

Clicks: 453
ID: 423
2018
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #1 of 36 articles by views in journal of bioscience and bioengineering

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Polyphenol oxidase (PPO) was extracted from Hass avocados and its physicochemical properties were analyzed. The optimum pH and temperature of the enzyme were pH 7.5 and 20°C. This PPO showed a high thermal stability, since 26% of the initial activity was retained by the enzyme after heating at 60°C for 40 min. Inhibition studies were performed using different chemical reagents, and the order in the inhibition efficiency was paeonol > 4-hydroxybenzaldehyde > β-cyclodextrin (β-CD). The first two inhibitors presented a non-competitive mechanism while the inhibition by β-CD results from a mixed type mechanism. Since the aqueous solubility of paeonol (a natural compound) is very low, the inclusion complex between this drug and β-CD was obtained in solution and solid state. The stoichiometry of the paeonol:β-CD complex was 1:1 and its ΔG° of formation was -26 kJ/mol. The complexation of paeonol by β-CD not only enhances the aqueous solubility and thermal stability of the drug, but also improves the in vitro inhibition efficiency against PPO. Colorimetric analysis on avocados pulp (in vivo) showed that the inclusion complex does not increase the inhibitory effect of paeonol, remaining practically unchanged. However, the formulation of paeonol:β-CD inclusion complex allows employing this compound as PPO inhibitor in aqueous solutions.
Reference Key
fuentes campo2018in vitro Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fuentes Campo, Anahí;Sancho, Matias I;Melo, Gisela;Dávila, Yamina A;Gasull, Estela;
Journal journal of bioscience and bioengineering
Year 2018
DOI
S1389-1723(18)30811-9
URL
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.