Dual-color oligo-FISH can reveal chromosomal variations and evolution in Oryza species.

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ID: 41402
2019
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Ranked #28 of 29 articles by views in The Plant journal : for cell and molecular biology

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Abstract
Fluorescence in situ hybridization using probes based on oligonucleotides (oligo-FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo-FISH probes that allow identification of each of the 12 pairs of chromosomes in rice (Oryza sativa). These two probes comprised 25,717 (green) and 25,215 (red) oligos (45 nucleotides), respectively, and generated 26 distinct FISH signals, which can be used as a barcode to uniquely label each of the 12 pairs of rice chromosomes. Standard karyotypes of rice were established using this system on both mitotic and meiotic chromosomes. Moreover, the dual-color oligo-FISH was used to characterize diverse chromosomal abnormalities. Oligo-FISH analyses using these probes in various wild Oryza species revealed that chromosomes from the AA, BB or CC genomes generated specific and intense signals similar to those in rice, while chromosomes with the EE genome generated less specific signals and the FF genome gave no signal. Together, the oligo-FISH probes we established can will be a powerful tool for studying chromosome variations and evolution in genus Oryza. This article is protected by copyright. All rights reserved.
Reference Key
liu2019dualcolorthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Liu, Xiaoyu;Sun, Shang;Wu, Ying;Zhou, Yong;Gu, Siwei;Yu, Hengxiu;Yi, Chuandeng;Gu, Minghong;Jiang, Jiming;Liu, Bao;Zhang, Tao;Gong, Zhiyun;
Journal The Plant journal : for cell and molecular biology
Year 2019
DOI
10.1111/tpj.14522
URL
Keywords Keywords not found

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