A triazole-based fluorescence probe for detecting Hg ions and its biological application.
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ID: 41397
2019
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Abstract
A new compound, ethyl 5-phenyl-2-(p-tolyl)-2H-1,2,3-triazole-4-carboxylate was successfully introduced and synthesized as a novel rhodamine B derivative named REPPC, and characterized by H nuclear magnetic resonance (NMR), C NMR, and high resolution mass spectrometry (HRMS). It showed an obvious fluorescence and UV-visible light absorption enhancement towards Hg ion without interference from common metal ions in N,N-dimethylformamide-H O (pH 7.4). The spirolactam ring moiety of rhodamine in REPPC was converted to the open-ring form generating a 1:1 complex with the intervention of a mercury ion, verified by electrospray ionization-mass spectroscopy testing and density functional theory calculation. REPPC was used to visualize the level of mercury ions in living HeLa cells with encouraging results.
| Reference Key |
liu2019aluminescence
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|---|---|
| Authors | Liu, Rong Qiang;Ding, Guo Hua;Li, Jian Ling;Feng, Hua Jie;He, Wen Ying;Wu, Lu Yong; |
| Journal | Luminescence : the journal of biological and chemical luminescence |
| Year | 2019 |
| DOI |
10.1002/bio.3705
|
| URL | |
| Keywords | Keywords not found |
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