A microscopic, biochemical, and molecular comparison of moderately resistant and susceptible Populus genotypes inoculated with Sphaerulina musiva.

Clicks: 341
ID: 38911
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #8 of 27 articles by views in Phytopathology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Sphaerulina musiva, the causal agent of Septoria leaf spot and stem canker, is responsible for mortality and yield loss in Populus plantations. Little is known about the mode of infection and the mechanisms of resistance in this pathosystem. To characterize these phenomena, microscopic, biochemical, and transcriptome comparisons were performed between leaves of moderately resistant and susceptible genotypes of Populus inoculated with S. musiva conidia. Using scanning electron, cryofracture and laser-scanning confocal microscopy, the infection and colonization of Populus leaves by S. musiva was examined across five time points (48 h, 96 h, 1 week, 2 weeks, and 3 weeks). The infection process was similar regardless of host genotype. Differences in host colonization between susceptible and moderately resistant genotypes were apparent by 1 week post-inoculation. However, the germination of conidia was greater on the susceptible than the moderately resistant genotype (P<0.008). Diaminobenzidine staining, a measure of hydrogen peroxide accumulation, was different (P<0.001) between the host genotypes by 2 weeks post-inoculation. Transcriptome differences between genotypes indicated that the speed and amplitude of the defense response was faster and more extensive in the moderately resistant genotype. The changes in gene expression support the microscopic and biochemical observations.
Reference Key
abraham2019aphytopathology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Abraham, Nivi;Chitampalam, Periasamy;Nelson, Berlin D;Sharma Poudel, Roshan;Chittem, Kishore;Borowiccz, Pawel;Brueggeman, Robert Saxon;Jain, Shalu;Leboldus, Jared;
Journal Phytopathology
Year 2019
DOI
10.1094/PHYTO-03-19-0105-R
URL
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.