Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells.

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ID: 3579
2019
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Abstract
Lipid-based nanoparticles, also called vesicles or liposomes, can be used as carriers for drugs or many types of biological macromolecules, including DNA and proteins. Efficiency and speed of cargo delivery are especially high for carrier vesicles that fuse with the cellular plasma membrane. This occurs for lipid mixture containing equal amounts of the cationic lipid DOTAP and a neutral lipid with an additional few percents of an aromatic substance. The fusion ability of such particles depends on lipid composition with phosphoethanolamine (PE) lipids favoring fusion and phosphatidyl-choline (PC) lipids endocytosis. Here, we examined the effects of temperature, ionic strength, osmolality, and pH on fusion efficiency of cationic liposomes with Chinese hamster ovary (CHO) cells. The phase state of liposomes was analyzed by small angle neutron scattering (SANS). Our results showed that PC containing lipid membranes were organized in the lamellar phase. Here, fusion efficiency depended on buffer conditions and remained vanishingly small at physiological conditions. In contrast, SANS indicated the coexistence of very small (~50 nm) objects with larger, most likely lamellar structures for PE containing lipid particles. The fusion of such particles to cell membranes occurred with very high efficiency at all buffer conditions. We hypothesize that the altered phase state resulted in a highly reduced energetic barrier against fusion.
Reference Key
kolainac2019influencenanomaterials Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Kolašinac, Rejhana;Jaksch, Sebastian;Dreissen, Georg;Braeutigam, Andrea;Merkel, Rudolf;Csiszár, Agnes;
Journal Nanomaterials
Year 2019
DOI
E1025
URL
Keywords
biofilm transcriptome salmonella typhimurium motility benzyl isothiocyanate real-world evidence treatment myelodysplastic syndrome high-risk clinical practice

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