Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages.

Clicks: 410
ID: 346
2019
Article Quality & Performance Metrics
Overall Quality Improving Quality
75.3 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment 🥈 High Quality
78.3 /100
Academic Rigor 78.0%
Novelty 72.0%
Clarity 85.0%
Key Strengths
  • Specific quantitative reporting of uptake (13%) and damage (25%)
  • Use of dimeric magnetite-gold structures which offer dual-modality potential
  • Clear focus on the interaction with the immune system (macrophages)
Areas for Improvement
  • Short incubation window (3 hours) may not reflect long-term bioaccumulation
  • Low uptake percentage suggests potential issues with targeting efficiency in vivo
  • Limited diversity in cell lines tested
AI Recommendations

Future studies should include a time-course analysis beyond 3 hours and compare these results with non-conjugated nanoparticles to isolate the effect of the PSMA ligand on macrophage internalization.

Enhanced v2.0 Analysis NISO/DORA Compliant
NISO/DORA Compliant
Research Integrity
⚡ Emerging
Topic Trend
2025 Relevance
Relevance
68%
Importance
76%
Authorship
Collaborative
Authors
7
Diversity
55%
Research Integrity
COPE Standards
Integrity
85%
Innovation
70%
Interdisciplinary Value
🔀 Cross-disciplinary
78%
Practical Impact Potential
Real-world Applications
74%
Enhanced Evaluation v2.0: Following NISO RP-25-2016, DORA 2025, and COPE assessment standards with 13 quality dimensions.
Abstract
We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.
Reference Key
pirutin2019visualization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pirutin, S K;Efremova, M V;Yusipovich, A I;Turovetskii, V B;Maksimov, G V;Druzhko, A B;Mazhuga, A G;
Journal Bulletin of experimental biology and medicine
Year 2019
DOI
10.1007/s10517-019-04356-9
URL
Keywords
Magnetite-gold nanoparticles Prostate-specific membrane antigen Cytotoxicity macrophages nanomedicine Fluorescence labeling Intracellular uptake

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