Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages.

Visualización y citotoxicidad de nanopartículas diméricas de magnetita-oro marcadas con fluorescencia y conjugadas con el antígeno prostático específico de membrana en macrófagos de ratón.

Clicks: 496
ID: 346
2019
Article Quality & Performance Metrics
Overall Quality Improving Quality
75.3 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment 🥈 High Quality
76.4 /100
Academic Rigor 78.0%
Novelty 72.0%
Clarity 85.0%
Key Strengths
  • Specific quantitative reporting of uptake (13%) and damage (25%)
  • Use of dimeric magnetite-gold structures which offer dual-modality potential
  • Clear focus on the interaction with the immune system (macrophages)
Areas for Improvement
  • Short incubation window (3 hours) may not reflect long-term bioaccumulation
  • Low uptake percentage suggests potential issues with targeting efficiency in vivo
  • Limited diversity in cell lines tested
AI Recommendations

Future studies should include a time-course analysis beyond 3 hours and compare these results with non-conjugated nanoparticles to isolate the effect of the PSMA ligand on macrophage internalization.

Enhanced v2.0 Analysis NISO/DORA Compliant
NISO/DORA Compliant
Research Integrity
⚡ Emerging
Topic Trend
2025 Relevance
Relevance
68%
Importance
76%
Authorship
Collaborative
Authors
7
Diversity
55%
Research Integrity
COPE Standards
Integrity
85%
Innovation
70%
Interdisciplinary Value
🔀 Cross-disciplinary
78%
Practical Impact Potential
Real-world Applications
74%
Enhanced Evaluation v2.0: Following NISO RP-25-2016, DORA 2025, and COPE assessment standards with 13 quality dimensions.
Readership in this journal
Emerging

Ranked #2 of 80 articles by views in Bulletin of experimental biology and medicine

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.
Demostramos la posibilidad de penetración de nanopartículas de magnetita-oro conjugadas con el antígeno prostático específico de membrana en macrófagos de ratón. Se halló que, tras una incubación de 3 horas con nanopartículas a una concentración de 15 mg/litro a 37 °C, estas solo se observaron en el 13 % de los macrófagos. En aproximadamente el 90 % de las células, las nanopartículas se detectaron en el citoplasma. En estas condiciones, se evidenció daño en la membrana en el 25 % de las células. Estos resultados deben tenerse en cuenta en el desarrollo y la aplicación futuros de nanomateriales con fines diagnósticos y terapéuticos en oncología.
Reference Key
pirutin2019visualization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Pirutin, S K;Efremova, M V;Yusipovich, A I;Turovetskii, V B;Maksimov, G V;Druzhko, A B;Mazhuga, A G;
Journal Bulletin of experimental biology and medicine
Year 2019
DOI
10.1007/s10517-019-04356-9
URL
Keywords
Magnetite-gold nanoparticles Prostate-specific membrane antigen Cytotoxicity macrophages nanomedicine Fluorescence labeling Intracellular uptake

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.