Gold-decorated magnetic nanoparticles modified with hairpin-shaped DNA for fluorometric discrimination of single-base mismatch DNA.

Clicks: 428
ID: 345
2019
Article Quality & Performance Metrics
Overall Quality Improving Quality
84.7 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment 🥈 High Quality
88.3 /100
Academic Rigor 92.0%
Novelty 82.0%
Clarity 90.0%
Key Strengths
  • Comprehensive material characterization using DLS, XRD, SQUID, and UV/Vis.
  • Innovative dual-fluorophore signaling mechanism (fluorescein and pyrene).
  • Excellent linear range (0.1 nM to 1.0 μM) for ssDNA detection.
Areas for Improvement
  • Specificity is primarily demonstrated for guanine mismatches; broader mismatch profiles could be explored.
  • Limited discussion on performance in complex biological matrices like whole blood or saliva.
  • The 2019 publication date means it does not address the most recent 2024-2025 trends in AI-integrated signal processing.
AI Recommendations

Future iterations should include data on the probe's stability over time and its performance in real-world clinical samples to enhance practical impact scores.

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NISO/DORA Compliant
Research Integrity
Highly Innovative
Trending Topic
High Impact
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2025 Relevance
Relevance
84%
Importance
86%
Authorship
Collaborative
Authors
6
Diversity
72%
Research Integrity
COPE Standards
Integrity
90%
Innovation
80%
Interdisciplinary Value
🌐 Highly Interdisciplinary
88%
Practical Impact Potential
Real-world Applications
83%
Enhanced Evaluation v2.0: Following NISO RP-25-2016, DORA 2025, and COPE assessment standards with 13 quality dimensions.
Abstract
The authors describe the use of gold-decorated magnetic nanoparticles (Au/MNPs) in discriminating DNA sequences with a single-base (guanine) mismatch. The Au/MNPs were characterized through dynamic light scattering, X-ray diffraction, superconducting quantum interference device, and UV/visible spectroscopy. They were then conjugated to a probe oligomer consisting of a hairpin-shaped DNA sequence carrying two signalling fluorophores: fluorescein at its 3' end and pyrene in the loop region. When interacting with the target DNA sequences, the hybridized probe-target duplex renders the pyrene signal (at excitation/emission wavelengths of 345/375 nm) either quenched or unquenched. Quenching (or nonquenching) of the pyrene fluorescence depends on the presence of a guanine (or a nonguanine) nucleotide at the designated polymorphic site. The linear range of hybridization in these Au/MNPs is from 0.1 nM to 1.0 μM of ssDNA. Conceivably, this system may serve as a single-nucleotide polymorphism probe. Graphical Abstract Schematic presentation of probe-conjugated Au/MNP preparation (upper panel) and working principle to discriminate DNA with or without single-base (guanine) mismatch sequences at the polymorphic sites (lower panel). Py denotes pyrene-hooked pyrrolocytidine; F denotes fluorescein.
Reference Key
lee2019gold-decorated Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lee, Mei-Hwa;Leu, Ching-Chich;Lin, Cheng-Chih;Tseng, Yu-Fan;Lin, Hung-Yin;Yang, Chia-Ning;
Journal Mikrochimica acta
Year 2019
DOI
10.1007/s00604-018-3192-9
URL
Keywords
Gold-decorated magnetic nanoparticles Hairpin DNA probe single-nucleotide polymorphism fluorescence quenching dna biosensor Single-base mismatch fluorometry

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