The growth improvement of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet using photoautotrophic micropropagation system

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ID: 33951
2016
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Abstract
To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.
Reference Key
sasongko2016theindonesian Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Aries Bagus Sasongko;Asruwaidah Fatumi;Ari Indrianto;
Journal indonesian journal of biotechnology
Year 2016
DOI
10.22146/ijbiotech.27167
URL
https://jurnal.ugm.ac.id/ijbiotech/article/download/27167/17814
Keywords
in vitro carbon dioxide plant growth grammatophyllym scriptum plantlet photoautotrophic micropropagation system

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