Agrobacterium-Mediated Stable Transformation of Medicinal Plant Andrographis paniculata Callus Expressing β-glucuronidase (GUS) Gene
Clicks: 329
ID: 33916
2013
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
72.5
/100
329 views
241 readers
Trending
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #39 of 60 articles by views in indonesian journal of biotechnology
Most read
Least read
Bar heights use a square-root scale.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
This study was carried out to establish a stable genetic transformation in callus culture of Andrographis paniculata mediated by Agrobacterium tumefaciens. The leaf disks of A. paniculata were infected with A. tumefaciens LBA4404 carrying a binary vector pCAMBIA1304 that contain β-glucuronidase (GUS) and hygromycin phosphotransferase (hpt) genes. The infection was conducted by dipping method for one hour, followed by co-cultivation in the dark for three days. To examine transient GUS expression, the co-cultivated leaf disks were assayed for β-glucuronidase activity and to obtain stable transformed callus, the co-cultivated leaf disks were selected on the callus induction medium which contain 20 mg/l hygromycin for selection. The transformed callus was periodically subcultured every three weeks into the fresh selection medium over the 15 weeks period. To test a stable transformation, the callus was subjected to PCR analysis for GUS gene detection. The results indicated that the co-cultivated leaf disks expressed GUS activity and proliferated to produce callus on the selective medium. Analysis of PCR on the transformed callus indicated the presence 976 bp fragment that confi rmed the presence of β-glucuronidase gene. These fi ndings imply that the β-glucuronidase was stably integrated into A. paniculata callus culture. Keywords: Andrographis paniculata, Agrobacterium tumefaciens, andrographollide, transformed callus, β-glucuronidase gene.
| Reference Key |
marwani2013agrobacteriummediatedindonesian
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Erly Marwani;Agustina Tangapo;Fenny Martha Dwivany; |
| Journal | indonesian journal of biotechnology |
| Year | 2013 |
| DOI |
10.22146/ijbiotech.7873
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.