Isolation, identification, and detection of ACC deaminase gene-encoding rhizobacteria from rhizosphere of stressed pineapple

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ID: 33857
2019
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Abstract
ACC deaminase is a microbial cytoplasmic enzyme that cleaves ACC, a precursor of ethylene, in stressed plant. The aims of this study are to isolate, identify, and detect the presence of ACC deaminase gene-encoding rhizobacteria from rhizospheric soil sample of pineapple which have been exposured by abiotic and biotic stress; herbicide, flooding, and Phytopthora spp. stress. A total of 49 rhizobacterial isolates were obtained and 7 of them were observed for their growth on DF medium containing 3 mM L -1 ACC. Four best growth isolates were selected for genomic DNA extraction. They were molecularly identified as Stenotrophomonas maltophilia (3), Burkholderia territorii (2A), Pseudomonas oryzihabitans (5B), and Bacillus tropicus (1E). A set of primers were constructed which are 105F- acd S 5’-TGCCAAGCGTGAAGACTGC-3’and 244R- acd S 5’-GGGTCTGGTTCGACTGGAT-3’ to amplify the ACC deaminase gene ( acd S). Based on melt peak curve analysis, four products appeared specific single peak at 86, 89, 87, and 89.5 o C indicating a single product was produced. In addition, Blast search showed that four products meet ACC deaminase feature and their acd S sequences are clustered into an ancestral group compared to those of many bacterial strains deposited in the GenBank. This result indicates that ACC deaminase gene-encoding rhizobacteria from pineapple plantation of tropical origin may affect the acd S sequences and may contribute to the stress tolerance for plant host.
Reference Key
jaya2019isolationindonesian Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Dori Kusuma Jaya;Giyanto Giyanto;Novik Nurhidayat;Sarjiya Antonius;
Journal indonesian journal of biotechnology
Year 2019
DOI
10.22146/ijbiotech.39018
URL
https://jurnal.ugm.ac.id/ijbiotech/article/download/39018/24699
Keywords
ethylene rhizobacteria acc deaminase acds gene environmental stress

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