Crude-oil biodegradation by indigenous hydrocarbon-degrading bacteria and their co-culture from port dickson, Malaysia

Clicks: 2
ID: 338196
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #81 of 82 articles by views in letters in applied microbiology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Crude oil contamination threatens environmental quality due to the persistence and toxicity of petroleum hydrocarbons. Bioremediation using indigenous microorganisms offers an effective and environmentally friendly solution. This study isolated and characterized indigenous hydrocarbon-degrading bacteria from crude oil-contaminated soil collected from a refinery in Port Dickson, Malaysia, and assessed their biodegradation capability individually and as a co-culture. Soil samples were enriched in Bushnell-Haas medium supplemented with crude oil as sole carbon source to isolate potential hydrocarbon-degrading bacteria. Among ten isolates, two superior growth and biosurfactant activity isolates were selected and identified as Klebsiella pneumoniae (isolate OB) and Pseudomonas aeruginosa (isolate SC) using 16S rRNA gene sequencing. Biodegradation efficiency was determined gravimetrically after 21 days, while changes in the residual crude oil profile were examined by GC-MS. Individual degradation efficiencies reached 39.56% for K. pneumoniae and 43.41% for P. aeruginosa, while their mixed co-culture achieved substantially higher efficiency (54.67%), indicating synergistic interactions. GC-MS analysis further supported the crude-oil degradation results, revealing alterations in the residual hydrocarbon profile, including reductions in selected hydrocarbon-associated compounds and changes in the relative abundance of individual components. These findings demonstrate that indigenous biosurfactant-producing bacteria, particularly in co-culture, reveal enhanced crude oil degradation.
Reference Key
openalex_W7225079530 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Muhammad Hazim Zulkifli, Helmi Wasoh, Mohamad Puad Abu, Mohd Noor Mat Isa, Sangkaran Pannerchelvan, Nor'Aini Abdul Rahman
Are you one of these authors? Claim your profile
Journal letters in applied microbiology
Year 2026
DOI
10.1093/lambio/ovag093
URL
https://doi.org/10.1093/lambio/ovag093
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.