The E3 ligase PpMBR2 targets PpABI5 for degradation and negatively regulates cold tolerance in peach fruit

Clicks: 5
ID: 337993
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #646 of 650 articles by views in Plant physiology and biochemistry : PPB

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 650 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abscisic acid (ABA) is known to alleviate chilling injury (CI) of peach fruit, but the molecular mechanisms is poorly understood. Here, a regulatory module that mediates peach fruit CI response at both transcriptional and post-translational levels was identified. ABA induced the gene expression and protein accumulation of abscisic acid-insensitive 5 (PpABI5). In turn, PpABI5 stimulated the expression of genes involved in proline and flavonoid biosynthesis, antioxidant defense, and fatty acid desaturation. Transient and ectopic overexpression of PpABI5 enhanced cold tolerance in peach and tomato fruit, accompanied by elevated proline and flavonoid biosynthesis, enhanced antioxidant capacity, and fatty acid desaturation. PpABI5 interacted with an E3 ligase MED25-binding RING-H2 protein2 (PpMBR2), which ubiquitinated PpABI5 at the specific site of K140, thereby modulated its degradation and attenuated PpABI5-mediated transactivation of target genes. PpMBR2-overexpression aggravated fruit CI, whereas PpMBR2-silencing produced the opposite effect. Furthermore, PpABI5 interacted with an ethylene response factor CRF3 (PpERF-CRF3), and this interaction enhanced their transcriptional regulation of target genes. Collectively, the results reveal a dynamic regulatory module PpMBR2-PpABI5-PpERF-CRF3 that governs cold tolerance in peach fruit by regulating proline and flavonoid biosynthesis, antioxidant defense and fatty acid desaturation. These findings advance our understanding of phytohormone crosstalk and site-specific ubiquitination modification in fruit CI.
Reference Key
openalex_W7225013191 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Wenhui Li, Yuan He, Mei Dai, Jixuan Cao, Jingying Shi, Zunyang Song
Are you one of these authors? Claim your profile
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag814
URL
https://doi.org/10.1093/plphys/kiag814
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.