Lysine biosynthesis integrates carbon availability and energy homeostasis under fluctuating CO2

Clicks: 15
ID: 332623
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #505 of 653 articles by views in Plant physiology and biochemistry : PPB

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 653 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Lysine metabolism connects amino acid biosynthesis with mitochondrial respiration through coordinated synthesis and degradation pathways that interface with the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain (mETC). The critical role of this pathway is underscored by the embryonic lethality of the Arabidopsis L,L-diaminopimelate aminotransferase mutant (dapat), which is defective in a key step of lysine biosynthesis. Hypomorphic dapat mutant plants exhibit profound metabolic reprogramming and a constitutive energy-limited phenotype under non-stress conditions, suggesting that lysine biosynthesis contributes to basal metabolic homeostasis. We therefore hypothesized that impaired lysine biosynthesis restricts metabolic flexibility, but that changes in CO2 availability may partially compensate through carbon and nitrogen remodeling. To test this, we performed comprehensive metabolite profiling of wild-type and dapat mutant plants under low-, ambient-, and elevated-CO2 conditions. Elevated CO2 (eCO2) induced broad reorganization of carbon and nitrogen metabolism in both genotypes, reducing photorespiratory and stress-associated amino acids and modulating key metabolic ratios, including glycine/serine and malate/aspartate, in a genotype- and time-dependent manner. Proteomics revealed DAPAT photosynthetic compensation under ambient CO2, with RuBisCO over-accumulation and suppressed TOR activity, as indicated by decreased RPS6 phosphorylation, which eCO2 restored in parallel with phenotypic rescue. These adjustments were associated with partial attenuation of the DAPAT metabolic imbalance. Our findings reveal functional connections among lysine biosynthesis, photorespiration, and CO2-responsive metabolic flexibility, demonstrating that lysine metabolism serves as a regulatory node linking amino acid homeostasis to carbon availability and respiratory adjustment. This provides mechanistic insight into how plants maintain energy balance under fluctuating CO2 environments.
Reference Key
openalex_W7222729249 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Débora Gonçalves Gouveia, Samantha Kurz, Wesley Elias Bhering Barrios, Philipp Westhoff, Laia Segura Broncano, Auxiliadora Oliveira Martins, Florencia Coronel, Elina Welchen, Daniel H. González, Alisdair Robert Fernie, Adriano Nunes‐Nesi, Andreas P.M. Weber, Wagner L. Araújo
Are you one of these authors? Claim your profile
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag765
URL
https://doi.org/10.1093/plphys/kiag765
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.