BandQuant: a web application for Western blot quantification

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ID: 331720
2026
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Abstract
Abstract Motivation Western blot quantification typically requires multiple tools and manual data handling, which is time-consuming and difficult to audit. Existing software is either tied to proprietary hardware or requires local installation, and none provides an integrated path from raw image to fold-change. Results We present BandQuant, a free, browser-based web application that integrates the entire Western blot quantification workflow into a single session. It combines automated band detection, rolling-ball background subtraction, housekeeping normalization, fold-change computation, and interactive bar-chart visualization in a single interface. A relational database binds experimental metadata to the image at upload, enabling automated normalization and cross-experiment queries without manual relabeling. BandQuant measurements showed high agreement with Fiji (Pearson r = 0.994), and analysis time was reduced by 65%. Availability and implementation BandQuant is freely available at https://www.tiaodaibao.com/?locale=en-US. Source code: https://github.com/wanhj-git/BandQuant. Archived release: https://zenodo.org/records/19453998.
Reference Key
openalex_W7220846526 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Muyang He, Shanglin Jin, Hongjian Wan, Chengfeng Zhang
Journal Bioinformatics advances
Year 2026
DOI
10.1093/bioadv/vbag292
URL
https://doi.org/10.1093/bioadv/vbag292
Keywords Keywords not found

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