Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu²⁺ and Biological Thiols in Aqueous Solution and Living Cells.
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ID: 33126
2016
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Abstract
A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu(2+) and biothiols. It was found that HNA can specifically bind to Cu(2+) with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu(2+) ensemble displayed a "turn-on" fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu(2+) to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu(2+) is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu(2+) to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay.
| Reference Key |
jia2016synthesissensors
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|---|---|
| Authors | Jia, Hongmin;Yang, Ming;Meng, Qingtao;He, Guangjie;Wang, Yue;Hu, Zhizhi;Zhang, Run;Zhang, Zhiqiang; |
| Journal | sensors |
| Year | 2016 |
| DOI |
10.3390/s16010079
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| URL | |
| Keywords | Keywords not found |
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